Methylomicrobium kenyense AMO1 poses as an alternative to the well-studied methanotroph Methylomicrobium alcaliphilum 20Z in the context of ectoine production, an osmoprotectant with a high market value. M. kenyense AMO1 can produce ectoine at lower salinity levels (3-5%), which has positive industrial implications. To date, the genetic basis of ectoine biosynthesis in M. kenyense AMO1 has not been explored. Therefore, the aim of this study was to identify possible regulators of the ectoine biosynthesis cluster in M. kenyense AMO1. Hybrid whole-genome sequencing was used to identify the ectABC operon in M. kenyense AMO1. Growth curves were performed using M. kenyense AMO1 in Nitrate Mineral Salts medium at 0%, 3%, 5% and 6% NaCl concentrations. Ectoine and RNA were extracted at mid-exponential phase of growth and ectoine was quantified by HPLC-UV, while RNA was used to evaluate the gene expression of ectC (ectoine synthase) and marR (transcriptional regulator) by RT-qPCR. A MarR-family transcriptional regulator was identified adjacent to the ectABC cluster, indicating a potential role in repressing ectoine biosynthesis. Ectoine concentration and ectC gene expression were confirmed to increase with increased salinity. Expression of the marR gene, which was predicted to be a repressor of the ectoine production, presented a variable gene expression profile. Compared to 0% salinity, marR expression was higher at all NaCl concentrations, where expression was lowest at 5%, and similar at 3% and 6%. Further studies are being carried out with marR knockout strains, to better evaluate its role on ectoine regulation.