The survival of zoonotic pathogens in agricultural soils amended with organic materials represents a critical preharvest food safety concern. While animal-derived amendments are widely used to improve soil health, their impact on pathogen survival and interactions with native soil microbiota under environmental conditions remains insufficiently understood. A controlled incubation study was conducted using sandy and clay soils collected from agricultural field plots at the Teagasc, Johnstown Castle Research Facility, Ireland. Soils were sieved (4 mm), homogenised, and subjected to four treatments: (i) soil alone, (ii) soil with compost, (iii) soil inoculated with pathogens, and (iv) soil with compost plus pathogens. The pathogen cocktail comprised Salmonella, Listeria monocytogenes, Campylobacter jejuni, E. coli O157:H7, or Clostridium sporogenes spores (~6 log₁₀ CFU or spores/g). Treatments were prepared in quadruplicate and incubated at 4 °C and 14 °C to represent typical Irish winter and summer conditions. Pathogen survival was monitored over 120 days using selective culture and enrichment methods. Concurrently, shifts in soil microbial community composition were assessed using 16S rRNA gene sequencing to evaluate interactions between indigenous and compost-derived microbiota. Preliminary results indicate distinct pathogen-specific survival dynamics. Salmonella and E. coli O157:H7 exhibited comparable survival, while Listeria monocytogenes declined more rapidly. Campylobacter jejuni showed a rapid reduction within the first week, whereas Clostridium sporogenes spores remained stable throughout the study period. Temperature and amendment type influenced survival patterns, suggesting a role for both environmental conditions and microbial interactions.