Introduction: Little is known about the impact of climate change on antimicrobial resistance. It’s predicted that Ireland will experience increased annual flooding due to heavy rainfall events, impacting agricultural lands, increasing the risk of infection due to Escherichia coli and other Enterobacterales. Methods: Agricultural sites (n = 8) were identified and soil samples were taken from flooded and non-flooded areas. Through enrichment, E. coli were selected using EMB agar. 16S rRNA PCR and sequencing was used to determine E.coli identity. Disk diffusion and minimum inhibitory concentration methods were used to test antibiotic susceptibility. To test biofilm formation, 96-well plates and crystal violet staining have been utilised. Escherichia coli isolates with antibiotic resistance underwent whole genome sequencing (Novogene). Results: More E.coli (n=47) were isolated from flooded than non-flooded soil (n=15). Almost all E.coli (n=14) from non-flooded soils were susceptible to all antibiotics investigated. In flooded soils, 11 isolates exhibited multi-drug resistance (ampicillin, tetracycline, ciprofloxacin, trimethoprim, sulphonamide, trimethoprim/sulfalomethoxazole), which has been determined to be plasmid mediated. In total 91% of E.coli isolated can form biofilm of varying degree. Conclusion: A greater number of E.coli were isolated from flooded than non-flooded soils, 47 and 15 respectively, suggesting flooded soils are potential reservoirs for E.coli. Escherichia coli from flooded soils displayed greater antibiotic resistance, including plasmid mediated multi-drug resistance. Escherichia coli from both soil types have the ability to form biofilms.