The phyla Actinobacteria (now Actinomycetota) and Myxobacteria (now Myxococcota) both contain important soil-dwelling organisms. Despite Actinomycetota being mostly Gram-positive and Myxococcota Gram-negative, they contain genera (Streptomyces and Myxococcus respectively), which have very similar biological properties. Both genera have huge genomes (often exceeding 10 Mb, with a high GC content), are predators of other bacteria, produce antibiotics, and have complex lifecycles involving differentiation into spores. In this paper, we conducted a comparative analysis of the genome sequences and the predatory activities of Streptomyces and Myxococcus strains (including novel isolates), with the objective of further categorising their antibiotic production. Soil dwelling bacteria were isolated from woodland using standard microbiology techniques. 16S rRNA gene sequencing confirmed the isolation of a novel Streptomyces sp.(strain C4A), with sequence identity to the nearest Streptomyces type strain (Streptomyces albidoflavus) of just 88.73%. Draft genome sequences were obtained and annotated using the prokaryotic genome annotation pipeline, Prokka. Pan-genomic analysis revealed a surprising degree of openness of the Streptomyces spp. pan-genome, and is different to other Actinomycetota, but similar to that of Myxococcus spp. The number of metabolites across 3 Streptomyces isolates are 6, 26 and 64, which came as an output using anti-SMASH. On corelating the openness of the pan-genome and predatory profiles of Streptomyces spp. isolates which were assayed by testing predatory activity against a range of plant pathogens, the results demonstrate a marked difference in predatory profile compared to those of Myxococcus xanthus, thereby making it any interesting avenue to explore for tackling antimicrobial resistance.