One of the significant challenges with wastewater monitoring is the lack of an optimized and standardized sample processing workflow from sample collection to RNA detection. The efficiency of viral concentration methods, sampling variability, viral genome stability and the sensitivity of the detection assay are some of the factors to consider while designing a testing strategy. Due to the highly heterogeneous nature and presence of inhibitors, one must tailor methods selected at each step to use wastewater and validate performance using appropriate controls.