Antimicrobial resistance (AMR) is a public health issue affecting many areas including agriculture. In farms, animal manure can contain antibiotic resistant genes, antibiotic resistant bacteria and undegraded antibiotics. Due to the use of animal manure as a fertiliser and its deposition on soil, there are fears that AMR could spread through the food chain from animals to the environment. Therefore, the impact of animal manure spreading on AMR needs to be investigated. This study aims to; take soil and animal manure samples before and after animal manure spreading; extract DNA and characterise the taxonomic and AMR gene profile using shotgun sequencing and isolate resistant bacteria to investigate multidrug resistance (MDR) and other resistance mechanisms. Methods: Animal manure samples were taken before spreading and soil samples taken before, 2 weeks, 1, 2 and 3 months after manure spreading. Bacterial DNA was extracted from soil and manure and sent for shotgun sequencing. Antibiotic resistant bacteria were isolated by direct inoculation onto antibiotic-containing media. Results: Shotgun sequencing data reflects microbial taxonomy and the presence and persistence of AMR genes in farm soil over a 3-month period. 30 MDR isolated colonies were identified with most belonging to the genus Pseudomonas. Conclusion: This work investigates the effects of animal manure use as a fertiliser on the spread of AMR on farms. Shotgun sequencing data will reveal the impact of animal manure spreading on the microbial taxonomic and AMR gene profile of soil in agricultural fields.