Antimicrobial resistance (AMR) is one of the most challenging global health and food security problems facing society. Left un-tackled, AMR is predicted to cause more than 10 million deaths and an 11% decrease in livestock production annually by 2050. The use of antimicrobials in the agricultural setting poses an increased risk in the transmission of antimicrobial resistance (AMR). Medically important antimicrobials are being used to treat/prevent animal infections and control crop spoilage. For these reasons, AMR can only be addressed and faced with the “One Health” approach. Despite several studies reporting on the impact of agricultural antimicrobial usage on soil, crops, wastewater treatment plants, and farm animals, there is a significant gap in our understanding of how air contributes to the transmission of AMR in these settings. The Environmental Protection Agency (EPA) gap analysis (2021) identified air and aerosols as the least investigated mode of transmission of antimicrobial-resistant organisms (ARO) and antimicrobial-resistant genes (ARG). This research investigates the role of aerosols in the transmission of AROs and ARGs in the farm. Using the AirPrep™ Cub Sampler-ACD210, optimal methods for bioaerosol recovery were evaluated. This is fundamental when studying samples of low bioburden. Air sampling volume, duration and locations were compared. Sample processing using filter-washing and sonication steps were explored. Processing methods were assessed using CFU counts, DNA quantification and quality measurements and 16s-rRNA bacteria, fungal(ITS) and human(GADPH) qPCR DNA levels. The data generated will enable the ResistAMR project to perform optimal sample processing to maximize the recovery of airborne DNA for future sequencing.