The rise of AMR poses a growing threat to human, animal, and environmental health. CPE are of particular concern as they are resistant to carbapenems, critically important antibiotics used to treat MDR and ESBL infections. Detection of CPE in bovine faeces suggests a potential environmental reservoir and risk of horizontal gene transfer of carbapenemase genes which can disseminate across bacterial species. This study aimed to detect and characterise CPE in bovine faecal samples through phenotypic, molecular, and proteomic analyses. Bovine faecal samples (n= 450) were screened using selective media and confirmatory carbapenemase detection tests. Phenotypic testing identified CPE in 13.7% or 62 (n = 450) of samples. Of these, 20 CPE isolates were selected for further analysis. MALDI-TOF MS STAR was used to identify hydrolysis of the b-lactam ring and polymerase chain reaction (PCR) with Sanger sequencing was employed to detect CPE genes. MALDI-TOF MS identified hydrolysis of carbapenems consistent with CPE in 45% (n = 20) of isolates, while PCR detected the CPE gene blaVIM in 6.25% (n = 16) of isolates.  The discrepancy between results was observed due to suspected loss of resistance in the isolates over time.  The bacteria once isolated from faeces did not display resistance for an extended period of time.   The detection of CPE genes in Irish cattle confirms the presence of clinically significant resistance in agricultural environments. These findings highlight the need for enhanced One Health surveillance, biosecurity measures, and antimicrobial stewardship to mitigate the spread of CPE and protect public health.