The highest burden of HPV and cervical cancer are observed in low- and middle-income countries such as Nigeria. Nigeria has a very diverse geographical and socio-cultural factors; thereby rendering regional studies necessary. Many socio-cultural contributory factors of cervical cancer are predominant in Northern Nigeria. Nonetheless, only a limited studies have characterised HPV strains in cervical cancer in this region. Thus, it is not yet clear, which HPV subtype is dominant/endemic. This study therefore, aimed to determine the strains of HPV in cervical biopsies in Adamawa State, Northern Nigeria. Fifty archived cervical cancer biopsies from 2016 – 2017 were retrieved at Histopathology Department of Modibbo Adama University Teaching Hospital, Yola. The tissues were stained using H&E. HPV genes were quantified using HPV-specific qPCR kit. Pre-malignant and malignant histologic changes were observed in all biopsies. HPV subtypes 16, 18, 35 and 39 were detected with the frequency of 14(23%), 17(27%), 11(18%) and 20(32%), respectively. Many of these biopsies contain infection with more than one strain of HPV. Cervical biopsies with squamous cell carcinomas were shown to have the highest infection (71%) with multiple HPV strains. This study revealed that HPV subtype 39 is the most frequently identified. Understanding the molecular characteristics of HPV in cervical cancer in Northern Nigeria is valuable for personalised interventions and reducing the burden of the disease in the region. HPV subtyping is recommended in locations before deploying vaccine intervention for appropriate prevention/management of HPV-associated cervical cancer.